NAN 604: Nanotechniques T, Th 1:00-2:15
References:
1. Krumeich, F., Properties of Electrons, Their Interactions With Matter and Applications to Electron Microscopy, ETH Zurich (PDF) 2. Xia,
Y.N. and Whitesides, G. M., Soft
lithography. Annual Review of Materials Science, 1998. 28: p. 153-184. (PDF)
3. Alessandrini,
A. and Facci, P., AFM: a versatile tool in
biophysics. Measurement Science & Technology, 2005. 16(6): p. R65-R92. (PDF)
4. Butt,
H. J., Cappella, B. and Kappl, M., Force
measurements with the atomic force microscope: Technique, interpretation and
applications. Surface Science Reports, 2005. 59(1-6): p. 1-152. (PDF)
5. Grier,
D. G., A revolution in optical
manipulation. Nature, 2003. 424(6950):
p. 810-816. (PDF)
6. Neuman,
K. C. and S. M. Block, Optical trapping.
Review of Scientific Instruments, 2004. 75(9):
p. 2787-2809. (PDF) 7. Vilfan, I. D., Lipfert, J., Koster, D. A., Lemay, S. G. and Dekker, N. H. , Magnetic Tweezers for Single-Molecule Experiments, Springer Handbook of Single-Molecule Biophysics, 2009 (PDF)
Presentation/report: (Presentations in class on Nov. 27 & 29; report due on Nov. 29) - Each student will give a timed, conference-style presentation of 10 min (plus time for questions after) - Presentation should include a cursory explanation of the technique plus a description of one (1) application from a journal article - The report should include a more complete description of the technique plus an in depth description of two (2) journal articles (one can be the one in your presentation) - Each student will focus on a technique not covered in class. Each student's subject may be chosen by him/her (and cleared by the instructor), but each will be unique - Some possible examples include (but are not limited to):
electrospinning SQUID
FRET Dip-pen nanolithography
mass spectrometry dynamic light scattering
raman spectroscopy electrophoresis/dielectrophoresis
Links:
| Topic |
Slides | Outline | Additional Links |
| beam sources |
videos: 1, 2
PDF | the physics of emission; source materials and defining characteristics; ion sources; why use them?; types and typical characteristics | electron diffraction experiment video | | vacuum technology | video
PDF | Positive displacement pumps: Rotary Vane, Rotary piston, Roots; Momentum transfer pumps: Turbomolecular, Diffusion; Entrapment pumps: Cryopump; Measuring vacuum pressure: Thermocouples, Ionization gauges | Kurt Lesker vacuum technical notes; Pump family tree; Pump operating ranges | | thin films | video
PDF | Evaporative Techniques: Thermal evaporation, Molecular Beam Epitaxy (MBE); “Glow-Discharge” Processes: Sputtering; Thickness & Rate Measurement; Gas-Phase Methods: Chemical Vapor Deposition (CVD); Liquid-Phase Methods: Electroplating, Spin coating | basic description of several deposition processes | | "hard" lithography | video
PDF | Photolithography: The basics, Considerations and solutions, A practical guide; Electron beam lithography: The basics, E-beam resist; Ion Milling; Templated growth | interactive pholith procedures, youtube lithography: 1, 2, 3 | | ion milling | video
PDF | Ion Milling; IBID | Reviews: 1, 2 | | soft lithography | video
PDF | Materials: Poly Dimethylsiloxane (PDMS); Microcontact Printing (μCP); Replica Molding (REM); Microtransfer Molding (μTM); Micromolding in Capillaries (MIMIC); Solvent-assisted Micromolding (SAMIM) | PDMS prep, a second paper from Whitesides on Soft Lith | | etching | video
PDF | Wet etching: Mechanism, Crystal Planes; Dry etching: ion sputtering, Reactive Ion Etching (RIE) | | | fabrication strategy | video
PDF | Objectives; The device; Fabrication; Strategy | Discussion paper (Sazonova) | | emerging techniques: DNA Origami | video
PDF | Introduction; Origami design basics; Applications: 2D shapes, 2D topographical designs, Particle patterning, 3D designs, Machines | DNA origami paper, TED Talk: Paul Rothemund | | crystallography/diffraction | | Introduction; Apparati; Diffraction: Derivation of Bragg’s Law, The unit cell, 1D vs 2D; Application: protein crystallography | X-ray diffraction handout, interactive tutorials, protein crystallization, Bravais lattices | | transmission electron microscopy | | Overview; Components: Source, Condenser Lens, Aperture, Sample, Objective Lens, Objective Aperture, Intermediate Lens/Projector, Imaging Screen | TEM Components, TEM simulator | | scanning electron microscopy | | Overview, Components, Sample interactions | SEM primers: 1, 2 | | scanning probe techniques 1: STM | | History; Overview: STM; STM Mechanism; Components of STM: probe (formation), scanners(piezoelectricity) | Introduction to STM | | scanning probe techniques 2: AFM | | Atomic Force Microscopy: Cantilevers, Quadrant Photo Diode, Contact mode imaging, Tapping mode imaging, Non-contact mode, Artifacts; Other imaging modes | AFM primer, AFM artifacts | | emerging techniques: superresolution microscopy | | Introduction; Review: diffraction limit; Super-resolution techniques; STimulated EmissionDepletion Microscopy, Photo-Activated Localization Microscopy, STochastic OpticalReconstruction Microscopy; Video: Inventing PALM | STED/STORM, PALM, Nature Method of the Year video | | STM atomic manipulation | | Introduction; Technique: Experimental Aspects, Lateral Manipulation, Vertical Manipulation; Examples; Video: Don Eigler | Review article, TEDx Talk: Don Eigler | | scanning probe manipulation | | Introduction; Force Spectroscopy: Cantilever mechanics, The Force Curve, Interpretation, Lateral Forces; Nanomanipulation: Case study- MWNT on graphite | MWNT on graphite papers: 1, 2, 3 | | molecular attachment techniques | | Introduction; Physical Attachment: Adsorption, Beam deposition; Chemical Attachment: Gold-thiolchemistry, Silanization; Biochemical Attachment: DNA labeling, Biotin-streptavidin, Dig-Antidig | EBID welding video, a Msc thesis on tip functionalization | | mems manipulation | | Introduction; Fabrication; Actuation: electrostatic, electrothermal; Operation; Application | Molhave Thesis | | optical tweezers | | Introduction; Trapping mechanism; Apparatus; Multiple trapping: Time-sharing, Holographictweezers; Applications | (see pdf links at top of page), videos: 1, 2, 3 | | magnetic tweezers | | Introduction; Apparatus; Stretching force; Twisting force; Variations on the theme | (see pdf links at top of page) | | emerging techniques: micro and nanofluidics | | Introduction; Fabrication; Microfluidic valves; Example: Mixer; Nanofluidics; Example: DNA imaging | Quake TedX talk |
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